- ￥1700 - 4000
- GeneTex
- 美国
- GTX112980
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX112980
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
0.58 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
SMAD4
- 抗体英文名：
SMAD4 antibody
- 抗体名：
SMAD4 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SMAD4 antibody (GTX112980) diluted at 1:1000 and competitor's antibody (sc-7966) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SMAD4 antibody detects SMAD4 protein by western blot analysis. Various whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SMAD4 antibody (GTX112980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
SMAD4 stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD4 antibody (GTX112980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using SMAD4 antibody (GTX112980)
SMAD4 antibody detects SMAD4 protein a tcytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% PFA at RT for 15 min.
Green: SMAD4 protein stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: SMAD4 protein stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Blue: Hoechst 33342 staining.
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SMAD4 stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Zheng S et al., Int J Med Sci 2018;15(Epub)
Huang SC et al., EMBO Mol Med 2017 (PMID:28993429)
Fang CY et al., PLoS One 2017 (PMID:28591207)
Khuman MW et al., Toxicology 2016 (PMID:27889505)
Chen HY et al., Antioxidants (Basel) 2021 (PMID:33451157)
Gu J et al., Evid Based Complement Alternat Med 2019 (PMID:31214266)
dingding2004 想找一个药物来阻断SMAD4 的作用。不知有没有做过的。 SMAD4 是在转录水平发挥作用的，如果有这种药物，加在要干预的细胞培养液中，可以发挥作用吗？ dingding2004 自己顶下啊 caoying207 唉，没想到2年以后，我也在找SMAD4阻断剂（抑制剂 ），前辈，你找到了吗？ skyzhuz 半
Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors
The signaling pathways mediated by the transforming growth factor-β (TGF-β) family of factors are implicated in a wide array of biological processes including cell differentiation and proliferation, determination of cell fate during embryogenesis
Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations
Juvenile polyposis (JP) is a rare dominantly inherited tumor predisposition syndrome, the typical lesion being a benign hamartomatous intestinal polyp with dilated crypts. Solitary juvenile polyps are relatively common in childhood, and appear
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