- ￥1700 - 4000
- GeneTex
- 美国
- GTX110558
- 2025年07月15日
- WB, ICC/IF, IHC-P, IP, ChIP assay
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human APE1. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX110558
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, ChIP assay
- 浓度：
0.34 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
APE1
- 抗体英文名：
APE1 antibody
- 抗体名：
APE1 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
APE1 antibody detects APE1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted by 1:1000.
Cross-linked ChIP was performed with 293T chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-APE1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MDR1 promoter.
APE1 antibody detects APE1 protein at cytoplasm, nucleus and nucleolus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
APE1 antibody (GTX110558) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
APE1 antibody immunoprecipitates APE1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of APE1 protein expressing A431 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of APE1 by 3 μg of APE1 antibody (GTX110558) 10% SDS-PAGE The immunoprecipitated APE1 protein was detected by APE1 antibody (GTX110558) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of PFA-fixed A549, using APE1(GTX110558) antibody at 1:200 dilution.
APE1 antibody detects APEX1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
APE1 antibody (GTX110558) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Martins MB et al., Mutagenesis 2021 (PMID:33740813)
Verena Hurst et al., Mol Biol Cell 2021 (PMID:34379448)
Ken Asada et al., Nucleic Acids Res 2021 (PMID:33928345)
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