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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human SHP1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX102864
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P, IHC-Fr, IP
- 浓度:
1.34 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
SHP1
- 抗体英文名:
SHP1 antibody
- 抗体名:
SHP1 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with SHP1 antibody (GTX102864) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
Various whole cell extracts (10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP1 antibody (GTX102865) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SHP1 antibody detects SHP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SHP1 stained by SHP1 antibody (GTX102864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP1 antibody detects SHP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
SHP1 stained by SHP1 antibody (GTX102864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP1 antibody (GTX102864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SHP1 antibody immunoprecipitates SHP1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SHP1 by 3 μg of SHP1 antibody (GTX102864) 7.5% SDS-PAGE The immunoprecipitated SHP1 protein was detected by SHP1 antibody (GTX102864) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX102864 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SHP1 antibody detects SHP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SHP1 stained by SHP1 antibody (GTX102864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP1 antibody detects SHP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SHP1 stained by SHP1 antibody (GTX102864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP1 antibody detects SHP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
SHP1 stained by SHP1 antibody (GTX102864) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Klaver EJ et al., PLoS One 2015 (PMID:25897665)
Liu BL et al., JCI Insight 2020 (PMID:32960817)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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