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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101506
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-Fr, FACS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
SOX2
- 抗体英文名:
SOX2 antibody
- 抗体名:
SOX2 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody (GTX101506) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Green: beta III Tubulin antibody [GT886] (GTX631830) diluted at 1:2000.
Red: Sox2 antibody (GTX101506) diluted at 1:250.
SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% PFA at RT for 15 min.
Green: SOX2 protein stained by SOX2 antibody (GTX101506) diluted at 1:200.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Immunofluorescence analysis of PFA-fixed Human ESC, using SOX2(GTX101506) antibody at 1:100 dilution.
Immunofluorescence analysis of PFA-fixed mouse ESC, using SOX2(GTX101506) antibody at 1:200 dilution.
Sample (30 μg of whole cell lysate)
A: GL261
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5). Red: Sox2 antibody (GTX101506) diluted at 1:500.
Blue: DAPI
Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101506)antibody at 1:50 dilution(green) or rabbit IgG (black).
Sample (20 μg of whole cell lysate )
A: A431 (GTX27909)
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (20 μg of whole cell lysate)
A: Human ESC
10% SDS PAGE
GTX101506 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of PFA-fixed human embryonic stem cell, using SOX2(GTX101506) antibody at 1:100 dilution.
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文献和实验Seleit A et al., Elife 2017 (PMID:28950935)
Uribe-Etxebarria V et al., Eur Cell Mater 2017 (PMID:29092089)
Chu PY et al., Cell Biosci 2022 (PMID:35941699)
Silvia T Erni et al., Front Cell Dev Biol 2021 (PMID:34485296)
Yan Z et al., Cell Death Dis 2021 (PMID:33608498)
Chiu YF et al., Sci Rep 2020 (PMID:32427884)
Lust K et al., Elife 2018 (PMID:29376827)
P矇rez Saturnino A et al., Development 2018 (PMID:30337377)
Lee CJ et al., Sci Rep 2018 (PMID:30510261)
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