- ￥1700 - 4000
- GeneTex
- 美国
- GTX100450
- 2025年07月11日
- WB, IHC-P
- Rabbit
- Human, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human ICAM1 / CD54. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX100450
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
ICAM1 / CD54
- 抗体英文名：
ICAM1 / CD54 antibody [N1C2]
- 抗体名：
ICAM1 / CD54 抗体 [N1C2]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
ICAM1 / CD54 antibody [N1C2] detects ICAM1 / CD54 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Green: ICAM1 / CD54 stained by ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ICAM1 / CD54 antibody [N1C2] (GTX100450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using ICAM1 / CD54(GTX100450) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Chang MC et al., J Formos Med Assoc 2018 (PMID:30558829)
Sun P et al., Biochim Biophys Acta 2017 (PMID:29175057)
Claude S et al., Mol Nutr Food Res 2014 (PMID:24425450)
Lee TH et al., PLoS One 2015 (PMID:25675397)
Koya T et al., Am J Physiol Heart Circ Physiol 2012 (PMID:22561298)
Chang TM et al., Int J Biol Sci 2023 (PMID:37056937)
Chang MC et al., Int Endod J 2021 (PMID:34420220)
Chang WC et al., Plants (Basel) 2021 (PMID:34371651)
Corgnac S et al., Cell Rep Med 2020 (PMID:33205076)
Huang FI et al., Front Oncol 2019 (PMID:31024851)
Sol? M et al., Biochim Biophys Acta Mol Basis Dis 2019 (PMID:31047972)
标本。 2. 加20ul 1 N HC I ,盖紧，上下混匀。 2 － 8 ℃ 放置60± 2 分钟。 3. 加20ul 1 N NaOH, 盖紧，上下混匀。 4. 即用，或放-20/-70 ℃ 保存3 天。计算结果时乘 以稀释倍数 50 。 （ 注意：不同的标本E2F1 的水平可能有较大差异，请根据实际情况灵活掌握稀释度） 5. 细胞培养上清或组织匀浆 10 倍稀释 (410ul 的标本
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
, 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml 上一篇：Antibody Conjugation Protocol 下一篇：Expanding Hybridoma Clones
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