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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Bcl-2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX100440
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Bcl-2
- 抗体英文名:
Bcl-2 antibody
- 抗体名:
Bcl-2 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100440) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100440) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bcl-2 antibody (GTX100440) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献和实验【求助】如果要证明Bcl-2和RARP活性,应该做什么实验?
eeflying 目前有两种化合物,分别为Bcl-2和PARP的抑制剂, 虽然这两个指标通常作为细胞凋亡的指标常用于分子生物实验, 但是,如何证明这两个分子被抑制的实验却第一次碰到, 请各位战友支招,我要直接检测哪个蛋白的WB 或者做什么实验合适? magichunter 用western检测bcl-2的含量变化,检测PARP活性裂解片段的含量变化以及原型态PARP的含量
Bcl-2蛋白是bcl-2 原癌基因的编码产物,是细胞存活促进因子,属膜整合蛋白,分子量为26kDa, 定位于线粒体、内质网和连续的核周膜。Bcl-2蛋白家族是一个特别的家族,成员中有些促进凋亡,如Bad、Bid 、Bax,有些成员阻止细胞凋亡,如Bcl-2、 Bcl-x、Bcl-w。Bcl-2能够阻止细胞色素c从线粒体释放到细胞质,从而抑制了细胞凋亡。
bcl-2 原癌基因(bcl-2 protooncogene)
人的B-淋巴细胞(B-cell lymphomas)中染色体易位激活的原癌基因。bcl即是B-淋巴细胞的英文缩写。该基因编码一种细胞质膜蛋白,能够抑制细胞的程序性死亡。bcl-2原癌基因与秀丽隐杆线虫的ced-9基因同源。
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