寰宇安泰生物(Uante)
产品编号 04942078001 | ROCHE
Dispase II (neutral protease, grade II)
产品信息:
Application
Dispase® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension.
Features and Benefits
• Rapid, effective, yet gentle agent that liberates cells with minimal cell damage
• Maintains cell membrane integrity
• Non-mammalian source - free of mycoplasma and animal virus contamination
• Extremely stable to influences of temperature, pH, and interference by serum components
• Easily inactivated by chelating agents or by dilution
• Delivers higher activity and convenience
General description
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
Activator: Optimal Ca2+ concentration is 2 mM. The enzyme preparations contain enough Ca2+ for optimal activity.
Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum.
Working concentration: 0.6 to 2.4 U/ml
Working solution: Preparation of stock and working solutions:
To produce a 10 mg/ml stock solution, dissolve the lyophilized Dispase® II enzyme in HEPES-buffered saline (50 mM HEPES/KOH pH 7.4, 150 mM NaCl). To produce the working solution, dilute the above stock solution with the culture medium for the isolated cells, at a final concentration of 0.6 to 2.4 U/ml. Note that concentrations higher than 2.4 U/ml are not recommended. For best results, filter the working solution using a 0.22 μm filter membrane.
Storage conditions (working solution): -15 to -25 °C
The reconstituted stock solution is stable at 2 to 8 °C for 2 weeks. For storage up to 2 months the stock solution should be frozen in aliquots. Avoid repeated freezing and thawing! The working solution diluted with PBS is stable at 2 to 8 °C for 3 days.
Specificity
Dispase is a nonspecific metalloprotease.
Unit Definition
One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 μmol (181 μg) tyrosine in 1 minute under assay conditions (pH 7.5, +37 °C).
A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where Dispase® concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase.
One unit of Roche Applied Science Dispase® equals 181 protease units (PU) measured as release of amino acids equivalent to 1 μg tyrosine per min and ml at pH 7.5 and +37 °C.
法律信息
Dispase is a registered trademark of Godo Shusei Co., Ltd.
重庆市寰宇安泰生物科技有限公司【ChongqingUante Biological Technology Co.,Ltd.】专注于生命科学科研试剂、耗材、仪器仪表等销售与科研技术服务。主营产品覆盖分子生物学、细胞生物学、免疫学、生物制药、诊断试剂研发、干细胞研究、美容抗衰等众多领域。代理或经销数十家知名品牌产品,诸如:SarePro、GEB、PALL、Sigma、Thermo Fisher、Lonza、StemCell、CST、Biolegend、BD等。将为广大科研用户在生命科学研究领域,提供高品质科研产品服务。公司秉承“诚信为本、服务至上”的管理理念,在未来的工作中,不断努力,奋发进取,力争成为您的科研物资战略合作优选伙伴!为生命科学领域的一线工作者提供最强有力的基础与支撑。