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- 详细信息
- 文献和实验
- 技术资料
- 宿主:
参见网站
- 抗原来源:
参见网站
- 库存:
大量
- 供应商:
赛信通(上海)生物试剂有限公司暨美国CST中国分公司
- 是否单克隆:
0
- 规格:
30 ml
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文献和实验. 3) Add 300μl blocking buffer to each well. Incubate 1 hour at R.T. 4) Aspirate and wash plate 4 times (as in step 2). 2. ELISA protocol 1) Standard/Sample: Serial dilute standard from 0.01μg/ml to zero in diluent. Add
SANDWICH ELISA FOR DETECTING SECRETE
. 9. Add 50 ul/well HP-streptavidin diluted accoridng to manufacturers instructions. Current dilution is 1:2000 in 1% BSA/PBS. Incubate 30 min at 37oC. 10. Wash 4X with PBS. 11. Wash 1X with Substrate buffer without substrate. 12. Add 200 ul
SANDWICH ELISA FOR DETECTING SECRETE
SANDWICH ELISA FOR DETECTING SECRETE Materials NUNC-Immuno Plate IIF. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin.
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