STEMium?-ACF is a serum-free medium designed for optimal growth of human embryonic stem cells and induced pluripotent stem cells under feeder-free conditions. It is a sterile, liquid medium containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/ 95% air. STEMium?-ACF is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human embryonic stem cells and induced pluripotent stem cells in vitro. This medium should be used in conjunction with BD Matrigel? for feeder-free culture condition.
Components
STEMium?-ACF consists of 500 ml of STEMium? basal medium and 10 ml of StemGS?-ACF 50X human pluripotent stem cell growth supplement (Cat. No. 5872).
Product Use
STEMium?-ACF is for research use only. It is not approved for human or animal use, or for application in in vitrodiagnostic procedures.
Storage
Store the basal medium and complete STEMium?-ACF at 4°C. Store StemGS? 50X at -20°C. Protect from light.