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文献和实验: rate of migration will depend on the strength of their net surface charges: :: The protein that carries more +ve charges
How Do I make an Antibody against a Peptides
If your peptide is synthesized with a terminal cysteine residue (i.e., a free sulfhydryl group, -SH), you can use any maleimide-activated carrier protein to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre
Method for Single-Cell Electroporation
Laboratories, Palo Alto, CA) plasmid pEGFP, which drives green fluorescent protein expression (GFP) with a strong CMV promoter. Single cells transfected with pEGFP expressed bright GFP within 12 h after electroporation, detectable by epifluorescence
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