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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human Apolipoprotein H / APOH Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50.00 µg/100.00 µg
| 规格: | 50.00 µg | 产品价格: | ¥1790.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥3220.0 |
蛋白名称:Apolipoprotein H蛋白, Apolipoprotein H protein
蛋白构建:A DNA sequence encoding the human APOH (NP_000033.2) (Met 1-Cys 345) was expressed, with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 96 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. 2. Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated human LDLR-his, The EC50 of biotinylated human LDLR-his is 0.35 μg/mL. 3.Immobilized human APOH-his at 2 μg/mL (100 μl/well) can bind biotinylated mouse LDLR-his, The EC50 of biotinylated mouse LDLR-his is 26 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gly 20
蛋白分子量:The recombinant human APOH consists of 337 amino acids and predictes a molecular mass of 37.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhAPOH is approximately 53 kDa due to different glycosylation.
蛋白NP号:NP_000033.2
蛋白氨基酸序列:Met1-Cys345
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Overview of Protein Microarrays
. Hu, S., Xie, Z., Onishi, A., Yu, X., Jiang, L., Lin, J., Rho, H.‐s., Woodard, C., Wang, H., and Jeong, J.‐S. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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