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- 2025年07月16日
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文献和实验Fastfilter Plasmid Midi Kit Spin Protocol
2. Pellet up to 30-50 ml bacteria in appropriate vessels by centrifugation at 3,500-5,000 x g for 10 min at room temperature. 3. Decant or aspirate medium and discard. To ensure that all traces of the medium are removed, use a clean paper towel to blot
Fastfilter Plasmid Maxi Kit Spin Protocol
DNA Maxi column into a clean 50 ml centrifuge tube. Add 6 ml sterile deionized water (or TE buffer) directly onto the column matrix. Allow column to sit 2 min at room temperature. Centrifuge at maxi speed(no more than 6,000 x g) for 5 min to elute DNA
RNA Isolation From Animal tissue or cell culture
empty, centrifuge the column for 2 min at full speed ($13,000 x g) to completely dry the HiBind® matrix. 11. Elution of RNA. Transfer the column to a clean 1.5 mL microfuge tube (not supplied with kit) and elute the RNA with 15-20 ul of DEPC-treated water
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