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文献和实验gases is available. Minimum carrier gas purity percentages: helium 99.9995, hydrogen 99.9995. 检查气瓶压力,确认载气、补充气、可燃气合适。载气纯度百分比最小应达到:氦气,99.995;氢气,99.995。 3. Clean the injector port, replace critical injection port seals, replace injection port liners
miRNA microarrays(McManus Lab)(芯片)
://www.ambion.com/techlib/prot/bp_12200.pdf) clean-up kit or by ethanol precipitation (http://www.ambion.com/techlib/misc/acetate_precip.pdf) (Note: Do not use a glycogen carrier). or 2) Purifying small RNA on a denaturing acrylimide gel
RNA Isolation From Animal tissue or cell culture
empty, centrifuge the column for 2 min at full speed ($13,000 x g) to completely dry the HiBind® matrix. 11. Elution of RNA. Transfer the column to a clean 1.5 mL microfuge tube (not supplied with kit) and elute the RNA with 15-20 ul of DEPC-treated water
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