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上海希言科学仪器有限公司
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文献和实验contains 0.5 µg/ml EtBr 1.Add 29 g Amberlite XAD-16 (Rohm and Hass) for 1 L of solution. 2.Store the solution for 12 hr at room temperature, shaking it intermittently. 3.Filter the solution through a Whatman No. 1 filter, and discard the filtrate
Isolation of DNA fragments using glass milk (GENE-CLEAN)
distilled water (not made up 100 ml)Filter sterileAdd a further 0.5 g sodium sulphite to ensure final solution is saturated.Store in dark at 4 ℃ for no longer than 2 mths.1.Melt agarose in NaI solution at 60℃2.Add 10 ul finebind and allow finebind to bind
Observation of living and plastic-embedded chick embryos
3- to 6-day chick embryo. Obtain an egg and clean scissors, forceps, a plastic spoon. First prepare a small petri dish with Howard Ringer's solution. Gently rotate egg, clean with 70% ethanol and set down for a few minutes to allow the embryo
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