- 询价
- Biotium
- 20467-100uL
- 美国
- 2025年08月23日
- ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- Donkey
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Alkaline Phosphatase Donkey anti-rabbit IgG (H+L), highly cross-adsorbed, 1 mg/mL 20467-100uL
- 靶点：
Rabbit IgG
- 浓度：
2 mg/mL
- 应用范围：
ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- 宿主：
Donkey
- 保质期：
6个月
- 目录编号：
20467-100uL
- 库存：
99
- 供应商：
Biotium
- 标记物：
Alkaline Phosphatase
- 克隆性：
多克隆
- 保存条件：
-20°C
- 形态：
Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution
This is a highly cross-adsorbed donkey anti-rabbit IgG (H+L) secondary antibody labeled with our bright and photostable CF® dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum.
- Highly cross-adsorbed for specific staining with minimal background
- Available with 18 bright and photostable CF® dyes
- Alkaline phosphatase, biotin, and HRP conjugates also available
- Suitable for western, immunofluorescence, and immunohistology in FFPE tissues
Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed
| Conjugation | Ex/Em | Size | Purchase |
|---|---|---|---|
| CF®350 | 347/448 nm | 50 uL (100 ug) | 20351-1 |
| 0.5 mL (1 mg) | 20351 | ||
| 1 mg | 20351-1mg | ||
| CF®405S | 404/431 nm | 50 uL (100 ug) | 20420-50uL |
| 0.5 mL (1 mg) | 20420-500uL | ||
| 1 mg | 20420-1mg | ||
| CF®430 | 426/498 nm | 50 uL (100 ug) | 20462-50uL |
| 0.5 mL (1 mg) | 20462-500uL | ||
| 1 mg | 20462-1mg | ||
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20015-1 |
| 0.5 mL (1 mg) | 20015 | ||
| 1 mg | 20015-1mg | ||
| CF®543 | 541/560 nm | 50 uL (100 ug) | 20308-1 |
| 0.5 mL (1 mg) | 20308 | ||
| 1 mg | 20308-1mg | ||
| CF®555 | 555/565 nm | 50 uL (100 ug) | 20038-1 |
| 0.5 mL (1 mg) | 20038 | ||
| 1 mg | 20038-1mg | ||
| CF®568 | 562/583 nm | 50 uL (100 ug) | 20098-1 |
| 0.5 mL (1 mg) | 20098 | ||
| 1 mg | 20098-1mg | ||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20152-1 |
| 0.5 mL (1 mg) | 20152 | ||
| 1 mg | 20152-1mg | ||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20125-1 |
| 0.5 mL (1 mg) | 20125 | ||
| 1 mg | 20125-1mg | ||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20178-1 |
| 0.5 mL (1 mg) | 20178 | ||
| 1 mg | 20178-1mg | ||
| CF®647 | 650/665 nm | 50 uL (100 ug) | 20047-1 |
| 0.5 mL (1 mg) | 20047 | ||
| 1 mg | 20047-1mg | ||
| CF®660R | 663/682 nm | 50 uL (100 ug) | 20389-50uL |
| 0.5 mL (1 mg) | 20389-500uL | ||
| 1 mg | 20389-1mg | ||
| CF®680 | 681/698 nm | 50 uL (100 ug) | 20418-50uL |
| 0.25 mL (500 ug) | 20418-250uL | ||
| CF®680R | 680/701 nm | 50 uL (100 ug) | 20195-1 |
| 0.5 mL (1 mg) | 20195 | ||
| CF®750 | 755/777 nm | 50 uL (100 ug) | 20298-1 |
| 0.25 mL (500 ug) | 20298 | ||
| CF®770 | 770/797 nm | 50 uL (100 ug) | 20484-50uL |
| 0.25 mL (500 ug) | 20484-250uL | ||
| CF®790 | 784/806 nm | 50 uL (100 ug) | 20344-50uL |
| Biotin | N/A | 50 uL (100 ug) | 20191-1 |
| 0.5 mL (1 mg) | 20191 | ||
| 1 mg | 20191-1mg | ||
| HRP | N/A | 100 uL (100 ug) | 20405-100uL |
| 1 mL (1 mg) | 20405-1mL | ||
| 1 mg | 20405-1mg | ||
| AP | N/A | 100 uL (100 ug) | 20467-100uL |
| 1 mL (1 mg) | 20467-1mL |
View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
CF® dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
CF is a registered trademark of Biotium, Inc.风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验H2O 1.84 g 柠檬酸 0.51 g DDW 100 ml 6. 显色液(现配现用)：底物Buffer 10 ml OPD 2 mg 30% H2O2 2 ml 7. 终止液 2 Mol/L H2SO4 8. 重组人TFAR19, HRP标记的羊抗人IgG9. ELISA板, Tip, 移液器，ELISA Reader(OD490 nm)，洗板机 操作步骤 1. 用包被Buffer稀释的重组人TFAR19(1 mg/ml)包被ELISA板, 100 ml/well, 37
% Tween 20 （3）封闭液： 3%BSA（用洗涤液配制） （4）酶标抗体的稀释：用封闭液稀释 （5）OPD底物Buffer：Na2HPO412H2O 1.84g；柠檬酸 0.51g ；DDW 100ml （6）显色液（现配现用）：底物Buffer 10ml；OPD 2mg；30% H2O2 2ml （7）终止液 2Mol/L H2SO48. 重组人TFAR19, HRP标记的羊抗人IgG9. ELISA板, Tip, 移液器，ELISA ，Reader（OD490nm），洗板机 操作
蛋白质印迹免疫分析（Western Blot Anglysis）
，3.3-二氨基联苯胺）配制：5mg DAB溶于10ml 柠檬酸buffer（0.01mol/L 柠檬酸2.6ml，0.02 mol/L Na2HPO4 17.39ml），加30% H2O2 10 μl（临用时现配）。 9．脱色液：甲醇250ml，冰醋酸100ml，加蒸馏水至1000ml。 10．氨基黑染色液（0.1%氨基黑-10B）：0.2g 氨基黑-10B 溶于200ml 脱色液中，充分搅拌溶解，滤纸过滤。 【操作步骤】
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