- ￥1700 - 4000
- GeneTex
- 美国
- GTX127309
- 2025年07月15日
- WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
- Rabbit
- Human, Mouse, Rat, Rabbit, Bovine
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Rabbit, Bovine
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX127309
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IP, ChIP assay
- 浓度：
1.17 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Knockdown/Knockout validation was supported by references (PMID:25428606)
- 抗体英文名：
HIF1 alpha antibody
- 抗体名：
HIF1 alpha 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney cancer.
HIF1 alpha antibody (GTX127309) dilution: 1:500.
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309).
Western blot analysis was performed using HIF1 alpha antibody (GTX127309) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% PFA at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200.
Blue: Hoechst 33342 staining.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.
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- 作者
- 内容
- 询问日期
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In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Immunohistochemical Detection of Tumour Hypoxia
In this chapter, we describe the use of immunohistochemical methods to detect hypoxia in tumour tissue sections, utilising antibodies specific for endogenous proteins hypoxia inducible factor 1 alpha (Hif1α) and glucose transporter 1 (Glut
marrow. The prime suspect: an adhesion molecule called alpha2 integrin. The molecule showed increased expression on memory cells, and its ligand is predominantly expressed in bone marrow tissue. Also, when the researchers blocked alpha2 integrin
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