- 询价
- Thermo scientific -fermentas
- ER1891
- 2025年10月06日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
阿拉丁试剂，充足现货，稳定批间差Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 10X Buffer Tango™ | ||||
| ER1891 | 300 u (5 u/µl) | 1.00 ml | ER1891 | |
| ER1892 | 1500 u (5 u/µl) | 1.00 ml | ER1892 |
| Recommended buffer for 100% activity | Optimal temperature | Enzyme activity in Fermentas buffers, % | Tango™ buffer for double digestion | |||||
|---|---|---|---|---|---|---|---|---|
| B (blue) 1X |
G (green) 1X |
O (orange) 1X |
R (red) 1X |
Tango™ (yellow) 1X / 2X |
||||
| Tango™ | 37°C | 0-20 | 0-20 | 0-20 | 50-100 | 100 | 50-100 | 1X or 2X |
Lambda DNA
0.7%琼脂糖
2 切割位点
33 mM Tris-acetate (pH 7.9, 37°C), 10 mM Mg-acetate, 66 mM K-acetate, 0.1 mg/ml BSA。
37°C酶切。
10 mM Tris-HCl (pH 7.5, 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA和50% (v/v)甘油。
Dcm: 可能重叠 – 不影响。
CpG: 完全重叠 – 阻止酶切。
EcoKI: 无重叠 – 不影响。
EcoBI: 无重叠 – 不影响。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| Dcm (CCWGG) | 5'...Cm5CW GG CGCGCm5CW GG ...3' |
不阻止酶切 |
| CpG | GGCGCGCC |
阻止酶切 |
| bp from the recognition site to fragment end | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 |
| 50-100 | ||||
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 2 | 0 | 0 | 0 | 0 | 0 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 0 | 0 | 0 | 0 | 0 | 0 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
during the extended incubation period. Crosses are made on petri dishes for specific purposes, as when shot asci are to be collected. But plates are more prone to contamination and desiccation than tubes, and less compact for incubation and storage. 2. Media
Diploid strains of asci, placed on suitable medium, sporulate and form four haploid spores/ascus, which may be arranged as tetrahedra, diamonds, or linearly. Strains forming linear arrays of spores in the ascus are rare, dissection
Hints and precautions for the care, feeding and breeding of Neurospora
with cross tubes because they result in more rapid water loss and are more prone to contamination during the extended incubation period. Crosses are made on petri dishes for specific purposes, as when shot asci are to be collected. But plates are more prone
技术资料暂无技术资料 索取技术资料
