- 询价
- Thermo scientific -fermentas
- ER1121
- 2025年10月05日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
|
| Catalog# | Size, concentration | Supplied with: | Certificate of Analysis | MSDS |
| 10X Buffer Tango™ | ||||
| ER1121 | 1000 u (10 u/µl) | 1.00 ml | ER1121 | |
| ER1122 | 5000 u (10 u/µl) | 2 x 1.00 ml | ER1122 | |
| ER1127 | 200 u (10 u/µl) | 1.00 ml |
| Recommended buffer for 100% activity | Optimal temperature | Enzyme activity in Fermentas buffers, % | Tango™ buffer for double digestion | |||||
|---|---|---|---|---|---|---|---|---|
| B (blue) 1X |
G (green) 1X |
O (orange) 1X |
R (red) 1X |
Tango™ (yellow) 1X / 2X |
||||
| Tango™ | 37°C | 50-100 | 20-50 | 0-20 | 0-20 | 100 | 0-20 | 1X |
Lambda DNA
1.4%琼脂糖
113 切割位点
33 mM Tris-acetate (pH 7.9, 37°C), 10 mM Mg-acetate, 66 mM K-acetate, 0.1 mg/ml BSA。
37°C酶切。
10 mM Tris-HCl (pH 7.4, 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA和50% (v/v)甘油。
Dcm: 无重叠 – 不影响。
CpG: 可能重叠 – 部分影响。
EcoKI: 无重叠 – 不影响。
EcoBI: 无重叠 – 不影响。
| Methylation type | Sequence | Cleavage effect |
|---|---|---|
| CpG | 5'...GTAm5C G ...3' |
部分影响 |
| CpG | 5'...m5C G TAm5C G ...3' |
阻止酶切 |
| bp from the recognition site to fragment end | ||||
|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 |
| 50-100 | ||||
| Lambda | ΦX174 | M13mp18/19 | pBR322 | puc18/19 | pUC57 |
|---|---|---|---|---|---|
| 113 | 11 | 19 | 3 | 3 | 3 |
| pTZ19R/U | pTZ57R | pBluescriptIIKS(-/+) | pBluescriptIISK(-/+) | pACYC177 | pACYC184 |
| 2 | 2 | 2 | 2 | 4 | 3 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Inverse PCR for PAC-end sequencing
of the insert. To increase the odds of getting an amplifiable product, we use two different enzymes (used singly!!) per pac end - NlaIII and RsaI for the SP6 end, and NlaIII and HinPI (or HhaI) for the T7 end. Primers PCYPAC2SP6L GCC GTC GAC ATT TAG
Inverse PCR For use with Snyder mTn-lacZ/LEU2 based mutagenesis
1 µl • Use either AciI, AluI, HaeIII, HpaII, RsaI or TaqI for Leu transposon libraries • 37 deg C/ >3 hours (or overnight) • 65 deg C/ 20 min III. Ligations (intramolecular, hopefully)
DOP-PCR Amplification of Whole Genomic DNA and Microchip-Based Capillary Electrophoresis
). In a more demanding protocol, called linker adaptor-PCR, RsaI restricted genomic DNA fragments are ligated to SmaI-cut pUC plasmid. Subsequently, the inserts are amplified by PCR using the universal M13/pUC sequencing and reverse sequencing
技术资料暂无技术资料 索取技术资料
