- ￥3975
- Agrisera
- 货号AS09 527
- 美国
- 2025年07月16日
- western blot (WB), immuprecipitation (IP), immunoc
- 兔
- 植物
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
兔抗拟南芥AGO1蛋白多克隆抗体
- 抗体英文名：
AGO1|argonaute 1
- 浓度：
见说明书
- 应用范围：
western blot (WB), immuprecipitation (IP), immunoc
- 宿主：
兔
- 适应物种：
植物
- 标记物：
无
- 克隆性：
多抗/单行
- 保存条件：
低温
- 亚型：
IgG
- 规格：
100 µg
供应商：上海经科化学科技有限公司
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兔抗拟南芥AGO1蛋白多克隆抗体介绍：
货 号：AS09 527
中文名称：兔抗拟南芥AGO1蛋白多克隆抗体
英文名称：AGO1|argonaute 1
应用：western blot (WB), immuprecipitation (IP), immunoc
规格：100 µg
价格：3975元
兔抗拟南芥AGO1蛋白多克隆抗体简介：
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| APPLICATION INFORMATION | ||
| recommended dilution | 1: 5000 - 1: 10 000 (WB) |
|
| expected | apparent MW | 116.4 | 130 kDa |
|
| confirmed reactivity | Arabidopsis thaliana, Nicotiana benthamiana |
|
| predicted reactivity | Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera |
|
| not reactive in | Chlamydomonas reinhardtii |
|
| additional information | AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot. |
|
| selected references | Minoia et al. (2014). Specific Argonautes Selectively Bind Small RNAs Derived from Potato Spindle Tuber Viroid and Attenuate Viroid Accumulation In Vivo. J Virol. 2014 Oct 15;88(20):11933-45. doi: 10.1128/JVI.01404-14. Epub 2014 Aug 6. | |
| application example 80 µg of Arabidopsis thaliana soluble total cell extract (extracted in 20 mMTris pH 7.5, 5mM MgCl2, 2.5mM DTT, 300 mM NaCl, 0.1% NP-40, 1% protease inhibitor MG132) was separated on 6% SDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 5% low-fat milk powder in TBS-TT (0.25% TWEEN20; 0.1% Triton-X) and probed with anti-AGO1 antibody (1:10 000, 1h) and secondary anti-rabbit (1:10000, 1 h) antibody (HRP conjugated, Santa Cruz(sc-2054)) in TBS-TT containing 5% low fat milk powder. Antibody incubationswere followed by washings in TBS-TT. All steps were performed at RT withagitation. Blots were developed for 5 min with ECL-PLUS detection reagent according the manufacturer's instructions (GE Healthcare). Exposure time was 5 seconds. Courtesy Dr. Ericka Havecker, University of Cambridge
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，小 RNAs (small RNAs，sRNA) 通过调控抗病相关基因表达发挥着重要作用。目前，植物胞外囊泡 sRNA 的分选机制仍不清晰。 2021 年 2 月 25 日，美国加州大学金海翎教授团队在 Nature Plants 发表研究论文 RNA-binding proteins contribute to small RNA loading in plant extracellular vesicles。该研究通过质谱分析对拟南芥胞外囊泡蛋白进行鉴定，发现 Argonaute 1 (AGO1
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