HA-tag标签抗体

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HA-tag标签抗体介绍：

货 号：AS12 2220

中文名称：HA-tag标签抗体

英文名称：HA-tag

应用：western blot (WB)

规格：100 µg

价格：5640元

HA-tag标签抗体简介：

PRODUCT INFORMATION background HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors. immunogen KLH-conjugated synthetic peptide: YPYDVPDYA host rabbit clonality polyclonal purity affinity purified serum format lyophilized quantity 100 µg reconstitution For reconstitution add 100 µl of sterile water. storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB) related products AS11 1771 | anti-His-tag | 6xHis mouse antibody AS09 601 | anti-c-Myc chicken antibody

APPLICATION INFORMATION
recommended dilution		1 : 5000 with standard ECL (WB)
expected | apparent MW		depends upon a MW of a protein which is HA-tagged
confirmed reactivity		HA-tag
not reactive in		no confirmed exceptions from predicted reactivity are currently known
additional information		to be added when available
selected references		to be added when available, antibody released in Febraury 2013.

application example

Total protein was extracted from WT and a transgenic line of Arabidopsis thaliana expressing a HA-tagged chloroplast protein. The extraction buffer contained 0.2M Tris-HCl pH 6.8, 10% Glycerol, 8% SDS, 20% bME. Proteins were separated on 11% SDS-PAGE, and blotted onto a nitrocellulose membrane. The blot was blocked with 5% non-fat dry milk in 1x TBS-T buffer for 1h at room temperature (RT) with agitation. The blot was incubated with the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted, the blot was rinsed briefly, and washed 3 times (5, 10 and 15 min) in TBS-T at RT with agitation. The blot was then incubated in a secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated; Agrisera, AS09 602, 1:10 000 dilution) for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer instructions. Signal was captured using the GE LAS500 instrument, with exposure time of 30 seconds.