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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
兔抗拟南芥AGO6蛋白多克隆抗体
- 抗体英文名:
AGO6|argonaute 6
- 浓度:
见说明书
- 应用范围:
western blot (WB)
- 宿主:
兔
- 适应物种:
植物
- 标记物:
无
- 克隆性:
多抗/单行
- 保存条件:
低温
- 亚型:
IgG
- 规格:
100 µg
供应商:上海经科化学科技有限公司
服务热线:400-0199-638
QQ:472482400(上海经科)
微信号:shjkchem
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兔抗拟南芥AGO6蛋白多克隆抗体介绍:
货 号:AS10 672
中文名称:兔抗拟南芥AGO6蛋白多克隆抗体
英文名称:AGO6|argonaute 6
应用:western blot (WB)
规格:100 µg
价格:5565元
兔抗拟南芥AGO6蛋白多克隆抗体简介:
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| APPLICATION INFORMATION | ||
| recommended dilution | 1: 1000 (WB) |
|
| expected | apparent MW | 116.4 | 99 |
|
| confirmed reactivity | Arabidopsis thaliana |
|
| predicted reactivity | Arabidopsis thaliana |
|
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment |
|
| additional information | AGO6 expression is cell specific. Seedlings can be used as a negative control. | |
| selected references | Havecker, E.R., Wallbridge, L.M., Hardcastle, T.J., Bush, M.S., Kelly, K.A., Dunn, R.M., Schwach, F., and Baulcombe, D.C. The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. manuscript revisions submitted, awaiting acceptance. |
|
Application example

360 µg/well of Arabidopsis thaliana protein extracted from leaf and saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (DayLight 800) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.
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文献和实验。你的目的蛋白和一抗的关系是什么,这点很重要。分析了这点之后,如果显色时间在1分钟之内,目的带可能是特异带。确信下面的带是特异的,建议换一抗,换其他封闭液。 llh刘丽华 目的蛋白和一抗的关系是什么?我不明白这事什么意思啊,我的目的蛋白RAC1蛋白啊,一抗是兔抗大鼠RAC1多克隆抗体啊! coffeeshine 目的蛋白是在什么宿主细胞里表达的? llh刘丽华 哦,是大鼠平滑肌细胞上表
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