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兔抗拟南芥AGO6蛋白多克隆抗体

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  • ¥5565
  • Agrisera
  • 货号AS10 672
  • 美国
  • 2025年07月15日
  • western blot (WB)
  • 植物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      兔抗拟南芥AGO6蛋白多克隆抗体

    • 抗体英文名

      AGO6|argonaute 6

    • 浓度

      见说明书

    • 应用范围

      western blot (WB)

    • 宿主

    • 适应物种

      植物

    • 标记物

    • 克隆性

      多抗/单行

    • 保存条件

      低温

    • 亚型

      IgG

    • 规格

      100 µg

    供应商:上海经科化学科技有限公司

    服务热线:400-0199-638


    QQ:472482400(上海经科)


    微信号:shjkchem

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    兔抗拟南芥AGO6蛋白多克隆抗体介绍:

    货 号:AS10 672

    中文名称:兔抗拟南芥AGO6蛋白多克隆抗体

    英文名称:AGO6|argonaute 6

    应用:western blot (WB)

    规格:100 µg

    价格:5565元

    兔抗拟南芥AGO6蛋白多克隆抗体简介:

    PRODUCT INFORMATION
    background

    AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

    immunogen

    KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 At2g32940 (O48771)

    host

    rabbit

    clonality

    polyclonal

    purity

    affinity purified serum

    format

    lyophilized

    quantity 100 µg
    reconstitution For reconstitution add 100 µg of sterile water.
    storage

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications

    western blot (WB)

    related products

    AS09 527 | AGO1 | argonaute 1

    AS09 617 | AGO4 argonaute 4, rabbit antibody

    AS10 673 | AGO9 | argonaute 9, rabbit antibody

    collection of antibodies to micro RNA

    Recommended secondary antibody for ECL detection

    additional information

    There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.

    AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6% denaturing gel to obtain good separation.

    If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.

    APPLICATION INFORMATION
    recommended dilution

    1: 1000 (WB)

    expected | apparent MW

    116.4 | 99

    confirmed reactivity

    Arabidopsis thaliana

    predicted reactivity

    Arabidopsis thaliana

    not reactive in

    no confirmed exceptions from predicted reactivity known in the moment

    additional information AGO6 expression is cell specific. Seedlings can be used as a negative control.
    selected references

    Havecker, E.R., Wallbridge, L.M., Hardcastle, T.J., Bush, M.S., Kelly, K.A., Dunn, R.M., Schwach, F., and Baulcombe, D.C. The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. manuscript revisions submitted, awaiting acceptance.


    Application example

    western blot using anti-AGO6 antibodies'

    360 µg/well of Arabidopsis thaliana protein extracted from leaf and saturated in 8M urea were separated on 15% SDS-PAGE and blotted for 1hour to 0.2 µm nitrocellulose at 100V using wet transfer system. Blots were blocked with 0.5% cold fish gelatin for 1hr at room temp with agitation. Blot was incubated in the primary antibody (anti-H3) at a dilution of 1:2500 for an hour at RT with agitation. The blots were washed with 3X 15min TBS-TT at RT with agitation. Blots as incubated in the secondary antibody (DayLight 800) 1:5000 dilution for 30min at RT with agitation and washed 1X with TBSTT for 15min, 1X with TBST for 15min before scanning with the ODyssey IRD scanner.

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