兔抗拟南芥DELLA蛋白RGA多克隆抗体

供应商：上海经科化学科技有限公司

服务热线：400-0199-638

QQ:472482400(上海经科）

微信号：shjkchem

活动：消费积分可换充值卡！

兔抗拟南芥DELLA蛋白RGA多克隆抗体介绍：

货 号：AS11 1630

中文名称：兔抗拟南芥DELLA蛋白RGA多克隆抗体

英文名称：RGA|DELLA protein RGA

应用：western blot (WB)

规格：100 µg

价格：3975元

兔抗拟南芥DELLA蛋白RGA多克隆抗体简介：

PRODUCT INFORMATION background DELLA protein RGA is a putative transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. It is a member of VHIID/DELLA regulatory family and is the most sensitive to GA application, compare to other DELLA proteins.Involved in fruit and flower development. Synonymes: GAI-related sequence, GRAS family protein 10, AtGRAS-10, Repressor on the ga1-3 mutant, Restoration of growth on ammonia protein 1. immunogen KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana Q9SLH3, At2g01570 host rabbit clonality polyclonal purity affinity purified serum format lyophilized quantity 50 µg reconstitution For reconstitution add 50 µl of sterile water. storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications western blot (WB) related products AS11 1631 | GAI | DELLA protein GAI, rabbit antibody AS06 194 | GA3 | gibberellic acid, rat antibody AS12 1870 | PIF3 | Phytochrome interacting factor 3 AS12 1860 | PIF4 | PHYTOCHROME INTERACTING FACTOR 4 AS11 1802 | RGA-like protein | DELLA protein RGL1 AS11 1803 | RGA-like protein 2 | DELLA protein RGL2 additional information to be added when available

APPLICATION INFORMATION
recommended dilution		1 : 1000 with standard ECL (WB)
expected | apparent MW		64 | 64 kDa
confirmed reactivity		Arabidopsis thaliana
predicted reactivity		only Arabidopsis thaliana
not reactive in		Triticum aestivum
additional information		RGA protein is prone to degradation therefore caution has to be taken during protein extraction.
selected references		Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016. Crocco et al. (2015). The transcriptional regulator BBX24 impairs DELLA activity to promote shade avoidance in Arabidopsis thaliana. Nat Commun. 2015 Feb 6;6:6202. doi: 10.1038/ncomms7202. Leone et al. (2014). To grow or defend? Low red : far-red ratios reduce jasmonate sensitivity in Arabidopsis seedlings by promoting DELLA degradation and increasing JAZ10 stability. New Phytol. 2014 Oct;204(2):355-67. doi: 10.1111/nph.12971. Epub 2014 Aug 7.

application example

40 µg of total protein from 5-d-old dark-grown Arabidopsis thaliana seedlings extracted with 50 mM Tris-HCl pH 7.5,, 10% glycerol, 150 mM NaCl, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail (Roche) were separated on 4-20 % SDS-PAGE and blotted 1h to PVDF. m: mock, P: paclobutyrazol, P+G: PAC+GAs. Blots were blocked with 2% blocking reagent (GE Healthcare) in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Femto reagent (Pierce) according to the manufacturers instructions. Exposure time was 20 seconds in a LAS-3000 Imager (Fuji).

Courtesy of Dr. David Alabadi, IBMCP (CSIC-UPV), Valencia, Spain

Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled in 2,5x Laemmli Buffer (WITH 60 Mm DTT final concentration) otherwise RGA protein will degrade. Plants were grown on 1/ MS for 15 days and were treated with 1 µM GA for 2 hours (GA+) or without hormone (GA-). Total protein extracts were denatured for 2 min. at 95°C and were separated on 10% SDS-PAGE and blotted overnight to PVDF using tank transfer. Blots were blocked for 1.5 h with TBS-T containing 5% low fat milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h 30min at RT with agitation. The antibody solution was decanted and the blot was washed 5 x 10min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 for 1h 30 min at RT with agitation. The blot was washed 6 x 10min in TBS-T at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds with West Femto (Pierce). ​