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上海希言科学仪器有限公司
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文献和实验of buffers prior to use. Storage Buffer for Column For 500ml 10mM Tris ph 7.5 5ml of 1M 0.3M NaCl150ml 1M or 60ml 2.5M 1mM EDTA 1ml of 0.5M 0.02% NaN3 1ml of 10% CREB Column - Purification Trial #1 Sample #5-C4-1-1 (pg.25)lysed in 4.5ml (3 vol
A280.Once absorbance has leveled off at zero,the column is sufficiently washed. 10)Elute column with the appropriate salt solutions. - When eluting SMase activity,elute: a)three times with (1 ml of)100 mM NaCl. b)two times with (0.7 ml)of 250 mM
in the column matrix!! 8)Wash column with 5 column volumes of lysis buffer containing no DMSO!! --> NOTE: DMSO strips proteins off of the coulumn! 9)Run a 40 ml salt gradient collecting 1 to 2 ml fractions: - Start at 0 mM NaCl and end with 500 mM NaCl. 10
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