半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin产品图
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半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)L

GALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
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  • 询价
  • Cloud-Clone
  • 标准曲线范围以随盒标准品梯度及该批次质检报告(COA)为准
  • 最低检出限以该批次质检报告(COA)标注为准
  • 美国
  • SCA321Hu
  • 2026年10月08日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商:

      安元生物科技(南京)有限公司

    • 检测范围:

      标准曲线范围以随盒标准品梯度及该批次质检报告(COA)为准

    • 检测方法:

      化学发光免疫法(CLIA)

    • 应用:

      科研用酶联免疫吸附测定

    • 适应物种:

      Homo sapiens (Human)

    • 标记物:

      HRP

    • 样本:

      Serum, plasma and other biological fluids

    • 灵敏度:

      最低检出限以该批次质检报告(COA)标注为准

    • 规格:

      48T/96T

    半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin 产品概述

    本48T/96T规格的半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin(GAL1,货号 SCA321Hu)由Cloud-Clone提供,运用CLIA完成Serum,、plasma、and、other、biological、fluids样本的目标物定量,方法成熟、结果可重复。

    半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin 检测原理

    本试剂盒基于CLIA原理:微孔板预先包被特异性捕获抗体/抗原,加入样本与标准品后形成免疫复合物,再经酶标记二抗结合并催化底物显色,吸光度值与目标物浓度成正比,通过标准曲线即可求得样本浓度。

    半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin 核心参数

    产品名称半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
    英文名称GAL1
    产品货号SCA321Hu
    品牌Cloud-Clone
    产品规格48T/96T
    检测方法CLIA
    适用样本Serum,、plasma、and、other、biological、fluids
    适用种属Homo sapiens (Human)
    样本用量50-100 μL/孔(以随盒技术资料为准)
    保存条件试剂盒 2-8℃ 避光保存,不得冷冻;复溶后的标准品请按要求分装并于 -20℃ 保存,避免反复冻融。
    保质期未开封 2-8℃ 条件下有效期 6 个月,具体以标签及质检报告(COA)为准。
    产地美国
    参考价格询价
    供应商安元生物科技(南京)有限公司

    GAL1 靶点背景与检测意义

    GAL1(NCBI Gene ID 3956,官方基因名 LGALS1)。The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.

    本试剂盒以 GAL1 为检测目标,适用于上述通路/功能相关的科研定量分析。

    半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin 适用样本与处理

    本试剂盒适用于 Serum,、plasma、and、other、biological、fluids。样本采集后请按下列要点处理:

    • 血清:采血后室温凝固 30 min,离心取上清,避免溶血;
    • 血浆:使用合适抗凝剂采集,及时离心分离;
    • 细胞培养上清:离心去除细胞碎片后取上清;
    • 组织匀浆:按质量体积比加入裂解/匀浆缓冲液,离心取上清。

    样本如不能立即检测,请分装后低温保存,避免反复冻融。

    半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin 操作流程

    1. 试剂与样本平衡至室温,按随盒技术资料配制标准品、洗涤液及工作液;
    2. 设置标准品孔、样本孔与空白孔,加入相应液体;
    3. 孵育后洗板,加入酶标试剂再次孵育并洗涤;
    4. 加入显色底物避光反应,加入终止液终止;
    5. 于酶标仪读取吸光度(常规 450 nm),计算结果。

    结果计算与标准曲线

    以标准品浓度为横坐标、吸光度值为纵坐标绘制标准曲线(推荐四参数或对数拟合),将样本 OD 值代入求得浓度;超出曲线上限的样本请稀释后复测,结果乘以稀释倍数。

    注意事项

    • 本品仅供科研使用,不可用于临床诊断;
    • 试剂请按 试剂盒 2-8℃ 避光保存,不得冷冻;复溶后的标准品请按要求分装并于 -20℃ 保存,避免反复冻融。;
    • 不同批号组分请勿混用,洗板应充分以保证重复性;
    • 显色时间需严格控制,终止后请及时读数。

    质量控制与随附文件

    产品出厂前经批间一致性、回收率与线性等质控项目检测,随盒提供质检报告(COA)与使用技术资料,检测范围与最低检出限以该批次 COA 标注为准。

    半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin 常见问题

    Q1:半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin适合检测哪些样本?
    A:适用于 Serum,、plasma、and、other、biological、fluids,其他样本类型建议先做预实验验证。

    Q2:适用种属有哪些?
    A:Homo sapiens (Human),跨种属使用请先进行验证。

    Q3:试剂盒如何保存、有效期多久?
    A:试剂盒 2-8℃ 避光保存,不得冷冻;复溶后的标准品请按要求分装并于 -20℃ 保存,避免反复冻融。未开封 2-8℃ 条件下有效期 6 个月,具体以标签及质检报告(COA)为准。

    参考文献(来源:PubMed)

    [1] Blidner AG et al. Glycosylation-driven programs coordinate immunoregulatory and pro-angiogenic functions of myeloid-derived suppressor cells. Immunity. 2025. PMID:40381622

    [2] Yu X et al. Galectin-1-Induced Tumor Associated Macrophages Repress Antitumor Immunity in Hepatocellular Carcinoma Through Recruitment of Tregs. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2025. PMID:39853961

    [3] Li Y et al. Hepatic GAL1 deficiency alleviates steatosis via WWP2-mediated PARP1 degradation and activation of the SIRT1-CPT1A pathway. Biochimica et biophysica acta. Molecular basis of disease. 2026. PMID:41951010

    靶点背景(来源:NCBI Gene)

    GAL1(NCBI Gene ID 3956,官方基因名 LGALS1,galectin 1)。The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.

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    图标文献和实验
    该产品被引用文献
    [1] Blidner AG et al. Glycosylation-driven programs coordinate immunoregulatory and pro-angiogenic functions of myeloid-derived suppressor cells. Immunity. 2025. PMID:40381622 [2] Yu X et al. Galectin-1-Induced Tumor Associated Macrophages Repress Antitumor Immunity in Hepatocellular Carcinoma Through Recruitment of Tregs. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2025. PMID:39853961 [3] Li Y et al. Hepatic GAL1 deficiency alleviates steatosis via WWP2-mediated PARP1 degradation and activation of the SIRT1-CPT1A pathway. Biochimica et biophysica acta. Molecular basis of disease. 2026. PMID:41951010
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    半乳糖凝集素1(GAL1)检测试剂盒(化学发光免疫分析法)LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
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