- ￥351
- hyclone
- 美国
- SH30870.01
- 2025年12月17日
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- 供应商：
hyclone
- CAS号：
SH30870.01
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文献和实验Isolation and Differentiation of Amnion-Derived Stem Cells (ADSCs)
Adipogenic induction Before adipogenic differentiation , ADSCs are rinsed extensively with PBS. ADSCs are exposed to adipogenic induction media (AIM) consisting of 10% FBS/DMEM, 500 μM IBMX, 1 μM dexamethasone, 10 μg/ml insulin, and 100 μM
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
of the transformation mixes (less then 2 ml total/plate) onto 24cm X 24cm Glu ura-his-trp- plate. There is no need to spin the cells or remove the PEG. The medium in these plates should be at least 0.6 cm thick, level, and free of bubbles. To achieve
complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
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