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文献和实验Problems about PhosphoProtein Purification
冷泉港有一本很经典的书,叫《蛋白质纯化与鉴定实验指南》。其中的附录7给出了用亚氨基乙二酸(铁螯合)Sepharose 6B分离磷酸肽的方法。后面有2篇参考文献,最重要的那篇因为发表时间太久远了(Muszynska, G., L. Andersson, and J. Porath. 1986. Selective adsorption of phosphoproteins on gel-immobilized ferric chelate. Biochemistry 25:6850-6853
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB
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