- ￥2270
- QIGEN
- 德国
- 180654
- 2025年10月29日
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文献和实验to shear DNA to lengths between 200 and 1000 basepairs being sure to keep samples ice cold. 5,� Centrifuge samples (part A, step 7) for 10 minutes at 13,000 rpm at 4�C, and detected the OD260 of the lysates. 6, Sonicated lysates
, as well as determining if the ChIP assay has errors. The kit also supplies PCR primers for both the positive and negative controls, facilitating the analysis of ChIP DNA by PCR. The column purified ChIP DNA can be directly used for downstream applications, either genome
2.Quantitate Protein Concentration Quantitate proteins in 15 ml aliquots of lysates (in duplicate)by using a Bradford assay (I have been using the Biorad kit),using BSA as standard.Don't forget to add 15 ml LB to each BSA sample and to the blank tube
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