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文献和实验cation. One pathway is to repair the cleaved site via nonhomologous endjoining (NHEJ) pathway, which applies only to the DSBs as SSNsare thought to be unable to induce NHEJ pathway. During NHEJmediatedrepair, insertion/deletion (indels
v468 Chapter 9 自身启动子报告基因验证转录靶点
of the canonical Wnt signaling pathway.However, b-catenin does not itself bind DNA, but functions via interaction with T-cell factor (TCF)/lymphoid-enhancing factor (LEF) transcription factors. These proteins contain a high-mobility group(HMG) box that binds DNA
Pools匹配,包含最多381个独特的TaqMan MicroRNA Assays,有效缩短了准备时间,并减少实验的不确定性。用Megaplex RT Primers反转录miRNA靶点及用Megaplex PreAmp Primers预扩增之后,TaqMan Universal PCR Master Mix可与每个反应简单混合,并移至TaqMan Array的八个上样口中之一,一个工作日内就能产生覆盖Sanger miRBase的综合数据集。你只需一台7900定量PCR仪和TLDA block就能完成
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