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文献和实验RNA in situ protocol for DNA and RNA probes
) to detect the hybridizing RNA in whole embryos. The major modification made was to increase the number of post-hybridization washes in hybridization buffer (50% deionized formamide, 5x SSC, 200 ug/ml tRNA, 100 ug/ml sonicated, boiled salmon sperm DNA
Two-hybrid analysis of genetic
of applying these methods to larger sets,we and others began devising ways to do so.Larger scale two hybrid approaches typically rely on interaction mating.In this method the protein fused to the DNA-binding domain (the bait)and the protein fused
【转帖】Top 100 proteomics publications
peptidome for cancer detection: spinning biologic trash into diagnostic gold. J Clin Invest 8. Li et al. (2006) An intron with a constitutive transport element is retained in a Tap messenger RNA. Nature 9. Miller et al. (2006) Semi-conservative DNA
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