- ￥5300
- GeneTex
- 美国
- GTX79247
- 2025年07月16日
- WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
- Rabbit
- Human, Mouse, Rat, Primate
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Primate
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Mouse
- 目录编号：
GTX79247
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody does not detect HSP86. The antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.
- 抗体英文名：
Hsp90 beta antibody
- 抗体名：
Hsp90 beta 抗体
- 规格：
100 μg
ICC/IF analysis of HepG2 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
ICC/IF analysis of U251 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
IHC-P analysis of human tonsil tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100
WB analysis of 50μg of the indicated whole cell lysates per well using GTX79247 HSP90 beta antibody.
Dilution : 1:1000
ICC/IF analysis of HeLa and NIH3T3 cells using GTX79247 HSP90 beta antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
FACS analysis of permeabilized, fixed HeLa cells using GTX79247 HSP90 beta antibody.
Blue histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 μg/ml incubated for 1 hour on ice
IHC-P analysis of human placenta tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
IHC-P analysis of human breast carcinoma tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100
WB analysis of mouse HSP86 (Hsp90 alpha) or HSP84 (Hsp90 beta) and protein using GTX22928 Hsp90 alpha antibody (Lane 1) or GTX79247 HSP90 beta antibody (Lane 2).
ICC/IF analysis of A2058 Cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
IP analysis of HeLa cell lysates using GTX79247 HSP90 beta antibody.
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000
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文献和实验Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
) antibodies and anti-beta 2 glycoprotein I (β2-GPI) antibodies. Other autoantibodies, such as those directed against anionic phospholipids, can also be assayed; however they are not of clinical significance. Participation in a quality assurance
。 如果beta-catenin抗体不好使，或者EGFP太大影响定位的话，还可以构建Flag标签类载体，转染细胞，加入药物，用抗Flag抗体免疫荧光检测，荧光显微镜下观察就好了。 wangjh28 但按照事先的推测，处理了以后会下调该基因的表达 我觉得科学不能有事先的推测的结果，这样容易受思维定势的影响，而忽略了新发现 dongreyuan 我打算用SAB的β-catenin（Ab-37）Antibody，说明
in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition of beta-mercaptoethanol to 0.1, glusulase
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