- ￥1700 - 4000
- GeneTex
- 美国
- GTX129426
- 2025年07月16日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the N-terminus region of human SP3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX129426
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
SP3
- 抗体英文名：
SP3 antibody
- 抗体名：
SP3 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and SP3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP3 antibody (GTX129426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
SP3 antibody detects SP3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: SP3 stained by SP3 antibody (GTX129426) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP3 antibody (GTX129426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP3 antibody (GTX129426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of SP3 protein from Jurkat nuclear extracts using 5 μg of SP3 antibody (GTX129426).
Western blot analysis was performed using SP3 antibody (GTX129426) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
SP3 antibody detects SP3 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SP3 antibody (GTX129426) dilution: 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SP3 antibody detects SP3 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP3 antibody (GTX129426) dilution: 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SP3 antibody detects SP3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SP3 stained by SP3 antibody (GTX129426) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SP3 antibody detects SP3 protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Jurkat nuclear extract
7.5 % SDS-PAGE
SP3 antibody (GTX129426) dilution: 1:1000
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文献和实验Lee J et al., Invest Ophthalmol Vis Sci 2020 (PMID:32721022)
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