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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NFkB p105(778-797aa WQVFDILNGKPYEPVFTSDD), identical to the related rat sequence, and different from the related human sequence by one amino acid.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Mouse
- 目录编号:
GTX12337
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
500 μg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
NFkB p105
- 抗体英文名:
NFkB p105 antibody
- 抗体名:
NFkB p105 抗体
- 规格:
100 μg
WB analysis of various samples using GTX12337 NFkB p105/p50 antibody.
Lane 1 : rat liver tissue lysate at 50ug
Lane 2 : rat spleen tissue lysate at 50ug
Lane 3 : rat testis tissue lysate at 50ug
Lane 4 : HeLa whole cell lysate at 40ug
Lane 5 : A431 whole cell lysate at 40ug
Lane 6 : Jurkat whole cell lysate at 40ug
Dilution : 0.5 μg/mL
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文献和实验renamed rel A) and p50 (recently renamed NFKB1) ( 3 , 4 ). Both of these sub-units belong to the rel famtly of transcription factors with homology in the amino terminus ( 5 , 6 ). Since the original identification of these factors
science_sky 各位前辈,我接下来的实验要检测NFkB通路的活化状况,现在在订购抗体时遇到了问题:我准备订购IκB Antibody 和Phospho-IκB来反映NFkB通路的活化状况,发现Cell Signaling Technology提供了IκBα ,IκBβ ,IκBε和IκB-ζ和相应的磷酸化抗体,我应该订购那种亚型的抗体呢?希望前辈给于指点。 00501101 你看你要做什么检测IκB抑制的是NF
是否特异性辨认正确蛋白。举例来说,表观遗传学重要蛋白 TET1,一般在细胞内的表达量较低, 蛋白生命周期也短, 往往不易侦测。这时可进一步使用过表达 TET1 的细胞裂解液作对照(下图 B),可看到抗体辨识的条带会明显增强,确定抗体辨识具有特异性。 另一个例子,黑色素瘤标记物-S100B 是钙结合蛋白,主要在中枢神经细胞表达,特别是星形胶质细胞。在黑色素瘤细胞和其他一些组织也发现 S100B 表达。为黑色素瘤的独立预后标志物。GeneTex 的 S100 beta antibody(GTX
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