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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
- 英文名:
TNF alpha protein
- 库存:
大量
- 供应商:
欣博盛
- 规格:
10 ug/50 ug
| 规格: | 10 ug | 产品价格: | ¥1606.5 |
|---|---|---|---|
| 规格: | 50 ug | 产品价格: | ¥3825.0 |
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文献和实验【求助】有什么办法可以阻滞LPS刺激的TNF-alpha分泌?
sonny_zan 我实验的关键步骤,现在就是不知道有什么办法可以阻滞LPS刺激后PBMC分泌TNF-alpha,考虑过用p38 MAPK抑制剂SB203580,后来查到一篇文献说SB203580对LPS刺激后p38 MAPK的活性影响甚微或无。 请各位大侠给点意见啦!我快急死了~~~ xiongran 拿抗体把PBMC表面的TLR4封闭掉试试看! pureclear 可以考虑
LABELING ALPHA-ACTININ WITH IATR
.Dialyze overnight against buffer 5 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 12.Determine the concentration of alpha-actinin using Lowry assay.Determine dye/protein molar ratio by diluting conjugate 1:40 (10μl in 400μl
Polymorphisms in the promoter of the tumor necrosis factor alpha (TNFα) gene have been reported to affect the transcription rate and the release of this cytokine. Among the best studied, the −238 and −308 polymorphisms have been associated
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