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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
over several days’ treatment to verify they are not actively dividing. If this is the case then phenol red in the medium remains red. It may also be necessary to perform a kill curve analysis of the Target ID library transfected cells if a change in susceptibility
protocol - Protocol for Annexin V-FITC Apoptosis Detection Kit (ab14085): 1. Incubation of cells with Annexin V-FITC 1) Induce apoptosis by desired method. 2) Collect 1-5 x 105 cells by centrifugation. 3) Resuspend cells
Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor
into the yeast strain. This red-shifted GFP-derivative has a higher emission intensity and longer fluorescence time than natural GFP (Straight et al . 1998). The laci later helps to stabilise the lacop array in yeast.; Insert a multimerised lacop array
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