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文献和实验Enzymatic Antibody Modification by Bacterial Transglutaminase
conjugates with improved properties for research or therapeutic use. As an example, we here provide a protocol for bacterial transglutaminase (BTGase)-mediated conjugation of cadaverine-derivatized substrates to an IgG1, resulting in stable bond formation
Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab)2, and Streptavidin
Colloidal gold probes, including protein A-, IgG-F(ab’ )2 -, and streptavidin-labeled gold particles, are useful tools for localization of antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different
the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
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