鸡肝癌细胞,LMH细胞

鸡肝癌细胞,LMH细胞基本信息
资源编号 hzCC268881
资源名称 LMH
种属 鸡肝癌细胞
提供形式 P1-2代冻存管
致病类型 hepatocellular carcinoma
安全等级 2
模式菌株 未知
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鸡肝癌细胞,LMH细胞培养方法
培养基 90%高糖DMEM+10%FBS
传代方法 Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution.
Allow the flasks to sit at room temperature (or incubate at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁生长
存储条件 50%高糖DMEM+40%FBS+10%DMSO 液氮
鸡肝癌细胞,LMH细胞详细说明
Strain Leghorn
Karyotype triploid; modal number = 116; six marker chromosomes
Derivation LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan.
Clinical Data Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
Receptor Expression estrogen receptor, positive
Tumorigenic Yes
Effects Yes, forms tumors in nude mice
Year of Origin 1981
描述 Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine.
The morphology of the cells is dendritic like.
The cells express glucose-6-phosphatase and weak canalicular ATPase activity.
The cells form tumors in athymic nude mice.
LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene.
The cell line is useful for transfection studies.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid.
Vessels prepared in this fashion may be used immediately.鸡肝癌细胞,LMH细胞HZC4138 上皮细胞转染试剂盒 EpiFectagen II?
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HZC3960 少突胶质细胞生长因子 OGS
HZC3822 神经细胞培养基 NM
HZC3957 神经元生长因子 NGS
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HZC4051 100X非必需氨基酸 NEAA
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HZC3006 仓鼠肺细胞 R 1610