人子宫平滑肌瘤细胞,SK-UT-1细胞基本信息
资源编号 hzCC337825
资源名称 SK-UT-1
种属 人子宫平滑肌瘤细胞
形态 epithelial
提供形式 P1-2代冻存管
致病类型 grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma
安全等级 2
模式菌株 未知
说明书 点击下载
产品图片 点击下载
人子宫平滑肌瘤细胞,SK-UT-1细胞培养方法
培养基 90%RPMI-1640+10%FBS
传代方法 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium Renewal: Twice per week
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁生长
存储条件 50% RPMI-1640+40%FBS+10%DMSO 液氮
人子宫平滑肌瘤细胞,SK-UT-1细胞详细说明
Age 75 years
Gender female
Ethnicity Caucasian
Karyotype (P8) hypodiploid to hyperdiploid
Clinical Data 75 years
Caucasian
female
Antigen Expression Blood Type B; Rh+
Tumorigenic Yes
Effects Yes, in nude mice
STR Profile Amelogenin X
CSF1PO 10,11
D13S317 13
D16S539 13,14
D5S818 10,11
D7S820 9,10
THO1 7
TPOX 8
vWA 15,16
Isoenzymes AK-1, 1
ES-D, 1
G6PD, B
Me-2, 1-2
PGM1, 1
PGM3, 1
Restrictions The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact email otd@mskcc.org
描述 The cells form spindle cell sarcomas.人子宫平滑肌瘤细胞,SK-UT-1细胞HZC3552 人尿道上皮细胞 HUC
HZC3160 人膀胱癌细胞 5637
HZC3303 人膀胱癌细胞 EJ
HZC3343 人膀胱癌细胞 T24-EGFP(CMV-EGFP慢病毒构建稳定株)
HZ-pc-h008 人膀胱癌组织源细胞 Bladder Cancer cell
HZC3553 人膀胱基质成纤维细胞 HBlSF
HZC3551 人膀胱平滑肌细胞 HBlSMC
HZC3550 人膀胱微血管内皮细胞 HBlMEC
HZC3170 人膀胱移行细胞癌 SW 780 [SW-780, SW780]
HZC3171 人膀胱移行细胞癌 J82
HZC3172 人膀胱移行细胞癌 UM-UC-3
HZC3410 人膀胱移行细胞癌 BIU-87
HZC3163 人膀胱移行细胞癌细胞 T24
HZC3002 人膀胱移行细胞乳头瘤细胞 RT4
HZC4052 人胚层检测试剂盒 HGL PCR
HZC4125 人胚层检测试剂盒 HGL PCR
HZC4218 人胚层检测试剂盒 HGL PCR
HZC3215 人胚肺成纤维细胞 HFL1
HZC3385 人胚肺成纤维细胞 MRC-5-EGFP+puro
HZC3120 人胚肺细胞 MRC-5
HZC3121 人胚肺细胞 WI-38
HZC3001 人胚肾细胞 293T/17
HZC3147 人胚肾细胞 2V6.11
HZC3148 人胚肾细胞 293 [HEK-293]
HZC3149 人胚肾细胞 293 Cells, low passage
HZC3150 人胚肾细胞 AAV-293
HZC3151 人胚肾细胞 293T
HZC3276 人胚肾细胞 293-L.P.
HZC3277 人胚肾细胞 GP-293
HZC3301 人胚肾细胞 293-mTLR5
HZC3377 人胚肾细胞 293XL-hTLR7
HZC3378 人胚肾细胞 293XL-hTLR9
HZC3278 人胚肾细胞-用于慢病毒包装 293FT
HZC3279 人胚肾细胞--用于慢病毒包装 293TN
HZC3326 人胚肾细胞--用于慢病毒包装 293LVT
HZC4027 人胚胎干细胞冻存液 StemCryo
HZC4028 人胚胎干细胞冻存液 StemCryo
HZC4072 人胚胎干细胞冻存液 StemCryo
HZC4073 人胚胎干细胞冻存液 StemCryo
HZC4054 人胚胎干细胞分离液 StemDS
HZC4071 人胚胎干细胞分离液 StemDS
HZC3997 人胚胎干细胞生长因子 50X StemGS
HZC3836 人胚胎干细胞无血清培养基 STEMium
HZC4068 人胚胎干细胞无血清培养基 STEMium
HZ-pc-h014 人皮肤癌组织源细胞 Skin Cancer cell