人子宫平滑肌瘤细胞,SK-UT-1细胞

人子宫平滑肌瘤细胞,SK-UT-1细胞基本信息
资源编号 hzCC337825
资源名称 SK-UT-1
种属 人子宫平滑肌瘤细胞
形态 epithelial
提供形式 P1-2代冻存管
致病类型 grade III,mesodermal tumor (mixed); consistent with leiomyosarcoma
安全等级 2
模式菌株 未知
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人子宫平滑肌瘤细胞,SK-UT-1细胞培养方法
培养基 90%RPMI-1640+10%FBS
传代方法 Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended. Medium Renewal: Twice per week
生长条件 95%空气+5%二氧化碳 37摄氏度
生长特性 贴壁生长
存储条件 50% RPMI-1640+40%FBS+10%DMSO 液氮
人子宫平滑肌瘤细胞,SK-UT-1细胞详细说明
Age 75 years
Gender female
Ethnicity Caucasian
Karyotype (P8) hypodiploid to hyperdiploid
Clinical Data 75 years
Caucasian
female
Antigen Expression Blood Type B; Rh+
Tumorigenic Yes
Effects Yes, in nude mice
STR Profile Amelogenin X
CSF1PO 10,11
D13S317 13
D16S539 13,14
D5S818 10,11
D7S820 9,10
THO1 7
TPOX 8
vWA 15,16
Isoenzymes AK-1, 1
ES-D, 1
G6PD, B
Me-2, 1-2
PGM1, 1
PGM3, 1
Restrictions The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact email otd@mskcc.org
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