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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
抗 RFP 标签多克隆抗体(兔源)
- 抗体英文名:
RFP tag Antibody pAb Rabbit
- 靶点:
RFP 标签
- 浓度:
0.5mg/mL(常规浓度,以官方说明书为准)
- 应用范围:
ELISA、免疫印迹、免疫组化、免疫沉淀
- 宿主:
兔(Rabbit)
- 适应物种:
通用
- 保质期:
- 20℃可稳定保存 半 年
- 抗原来源:
RFP 标签合成肽
- 级别:
科研级
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 标记物:
无(Unconjugated)
- 克隆性:
多克隆(Polyclonal)
- 保存条件:
-20℃,可稳定保存 半 年
- 形态:
液体(Liquid)
- 亚型:
Rabbit IgG
- 免疫原:
RFP 标签合成肽
- 规格:
40μg
| Specificity | We RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange.The antibody does not react with GFP or GFP-tagged proteins. |
| Host Species | Rabbit |
| Immunogen | RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Affinity chromatography |
| Isotype | Rabbit IgG |
| Clonality | Polyclonal |
| Clone ID | Not applicable |
| Target Background | Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems.RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone. |
| Synonyms | Rabbit Anti RFP-tag pAb;Anti RFP-tag pAb |
RFP-tag 抗体、RFP 标签抗体、兔源 RFP-tag 抗体、多克隆 RFP-tag 抗体、抗 RFP-tag 抗体、标签抗体、WB 抗体、IHC 抗体、IF 抗体、ELISA 抗体
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文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
with a small protein tag (e.g. Histidine-tag, c-Myc, FLAG or hemaglutinin). GFP, the most widely used labelling tag in cell biology is rarely used for biochemical analyses although various mono- and polyclonal antibodies are available (Cristea et al., 2005
应用。用GST融合表达系统表达外源基因时,需要对融合表达的产物进行检测和纯化,这里面就包括了GST标签抗体的应用,如果选择一个最具性价比的GST抗体显得尤为重要。首先需要考虑抗体的来源,比如小鼠源的单克隆抗体还是兔源的多克隆抗体,一般来说,单抗的特异性、稳定性以及效价都要优于多克隆抗体。EarthOx的GST标签抗体――Anti-GST Tag Monoclonal Antibody [GST.B6](货号#E022040)是来源于小鼠的单克隆抗体,克隆号为GST.B6,免疫原为GST多肽 。
Studies of the Ubiquitin Proteasome System
from other investigators) Transfection efficiency control plasmid, e.g., GFP, RFP, β‐galactosidase 2× HEPES‐buffered saline (HeBS; see recipe ) Anti– tag epitope antibody or anti‐substrate
技术资料




