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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
抗 V5 标签多克隆抗体(兔源)
- 抗体英文名:
V5 tag Antibody pAb Rabbit
- 靶点:
V5 标签
- 浓度:
0.5mg/mL(常规浓度,以官方说明书为准)
- 应用范围:
ELISA、免疫印迹、免疫组化、免疫沉淀
- 宿主:
兔(Rabbit)
- 适应物种:
通用
- 保质期:
- 20℃可稳定保存 半 年
- 抗原来源:
V5 标签合成肽
- 级别:
科研级
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 标记物:
无(Unconjugated)
- 克隆性:
多克隆(Polyclonal)
- 保存条件:
-20℃,可稳定保存 半 年
- 形态:
液体(Liquid)
- 亚型:
Rabbit IgG
- 免疫原:
V5 标签合成肽
- 规格:
40μg
| Specificity | We Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins. |
| Host Species | Rabbit |
| Immunogen | V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Affinity chromatography |
| Isotype | Rabbit IgG |
| Clonality | Polyclonal |
| Clone ID | Not applicable |
| Target Background | The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope. |
| Synonyms | Rabbit Anti-V5-tag pAb;Anti-V5-tag |
V5-tag 抗体、V5 标签抗体、兔源 V5-tag 抗体、多克隆 V5-tag 抗体、抗 V5-tag 抗体、标签抗体、WB 抗体、IHC 抗体、IF 抗体、ELISA 抗体
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
). 9. Secondary antibody to use with goat polyclonal reagent: donkeyanti-goat IgG-horseradish peroxidase (HRP) (sc-2056;Santa Cruz Biotechnology). 10. Secondary antibody to use with rabbit polyclonal reagent: donkey anti-rabbit IgG-HRP (#NA934V, GE
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
consists of 5% (w/v) dried skim milk in Trisbufferedsaline/Tween-20 (TBS-T) buffer (50 mM Tris-HCl,pH 7.5, 150 mM NaCl, and 0.05% (v/v) Tween-20). 4. All antibodies are diluted 1:1,000 in 1% (w/v) dried skim milkin TBS-T, except the CRMP2 antibody (1:250
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