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- 中国/美国
- xy-C817Mu01
- 2025年11月18日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Recombinant Thiosulfate Sulfurtransferase (TST)
- 库存：
1000
- 供应商：
上海信裕
硫代硫酸盐转硫酶(TST)重组蛋白
Recombinant Thiosulfate Sulfurtransferase (TST)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | RPC817Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 37.0kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Val2~Ala297 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
SEQUENCES
The sequence of the target protein is listed below.
VHQVLYRAL VSTKWLAESI RSGRLGPSLR VLDASWYSPG TRQARKEYQE RHVPGASFFD IEECRDTTSP YEMMLPSEAH FGDYVGNLGI SNDTHVVVYD GDDLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLNLS LLKTYEQVLE NLQSKRFQLV DSRAQGRYLG TQPEPDIVGL DSGHIRGSVN MPFMDFLTKD GFEKSPEELR AIFQDKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPETRVS QGKSGKA
VHQVLYRAL VSTKWLAESI RSGRLGPSLR VLDASWYSPG TRQARKEYQE RHVPGASFFD IEECRDTTSP YEMMLPSEAH FGDYVGNLGI SNDTHVVVYD GDDLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLNLS LLKTYEQVLE NLQSKRFQLV DSRAQGRYLG TQPEPDIVGL DSGHIRGSVN MPFMDFLTKD GFEKSPEELR AIFQDKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPETRVS QGKSGKA
硫代硫酸盐转硫酶(TST)重组蛋白 USAGE
Reconstitute in sterile ddH2O.
硫代硫酸盐转硫酶(TST)重组蛋白 STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
硫代硫酸盐转硫酶(TST)重组蛋白 About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
型的蛋白，或进行高解析度的结构分析，愈来愈多的实验组选择哺乳类表达系统来生产融合蛋白。其原因为哺乳动物细胞生产出的重组蛋白与人类蛋白十分相似，有较完整的蛋白折叠、组合及翻译后修饰[3]。因此，愈来愈多科学家们更加关注于—如何从哺乳类细胞中高效的纯化目标蛋白。1 亲和标签的选择首先，需考虑选择合适的亲和标签。常用于哺乳动物表达系统的亲和标签有 His-tag、FLAG-tag、GST-tag及 Strep-tag® 等[4-12]，整理于下表：（点击放大查看）这些亲和标签在使用上各有千秋。使用
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硫代硫酸盐转硫酶(TST)重组蛋白
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