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- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Trimethoprim sulfate
- 库存：
大量
- 供应商：
欣博盛
- 规格：
100 mg
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文献和实验Resistance to Tetracyclines, Macrolides, Trimethoprim and Sulfonamides
Tetracyclines are antimicrobial agents that interact with bacterial ribosomes and block protein synthesis. They have activity against a wide range of gram positive, gram-negative, anaerobic and aerobic bacteria, cell-wall free myco plasmas
Metabolic Labeling with Sulfate
of proteins make it possible to determine where a protein normally resides or to follow its transport through the cell. One such modification is addition of sulfate either to tyrosine residues or to carbohydrate side chains. Labeling studies with [35 S
Ammonium Sulfate Binding Assay for Cells
1. Resuspend cells (vegetative or developed) in phosphate buffer at 1 x 10 8 /ml . 2. Add 50 ul cells to 50 ul 20 nM 3H-cAMP in 5-10 mM DTT (for background binding, add same amount of hot cAMP plus 10 uM cold cAMP) in 1.5 ml eppendorf tube
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