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- Adipogen
- 瑞士
- CDX-C0241-G025
- 2025年07月15日
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- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Copper(II) tartrate hydrate
- 库存：
大量
- 供应商：
欣博盛
- 规格：
25 g
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文献和实验Bicinchoninic Acid (BCA) Protein Assay (Smith)
below. Principle BCA serves the purpose of the Folin reagent in the Lowry assay,namely to react with complexes between copper ions and peptide bonds to produce a purple end product.The advantage of BCA is that the reagent is fairly stable under alkaline conditions
,with maximum transmission in the region of 450 nm.Glass or polystyrene (cheap)cuvettes may be used.ProcedureReagentA formula for biuret reagent is 2.25 gm Sodium potassium tartrate (f.w.282.22),0.75 gm Copper sulfate x 5 H2O (f.w.249.68),1.25 gm Potassium
)].Under alkaline conditions,copper complexes with protein.When folin phenol reagent (phospho-molybdic-phosphotungstic reagent)is added,the Folin-phenol reagent binds to the protein.Bound reagent is slowly reduced and changes color from yellow to blue.While widely
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