- ￥6100
- GeneTex
- 美国
- GTX60833
- 2025年07月15日
- WB, ICC/IF, ChIP assay
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the α and γ isoforms.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60833
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ChIP assay
- 浓度：
2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
pan-HP1
- 抗体英文名：
pan-HP1 antibody - ChIP grade
- 抗体名：
pan-HP1 抗体 - ChIP grade
- 规格：
50 μg
ICC/IF analysis of 4% PFA fixed HeLa cells using GTX60833 pan-HP1 antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500
WB analysis of nuclear extracts (20 μg) from HeLa cells using GTX60833 pan-HP1 antibody - ChIP grade.
Dilution : 1:1,000
ChIP analysis of NIH3T3 cells using GTX60833 pan-HP1 antibody - ChIP grade. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. This figure shows the relative enrichment as compared to a no antibody negative control ChIP.
Antibody amount : 4μg
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文献和实验Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
Characterization and Quality Control of Antibodies Used in ChIP Assays
, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Abstract Table of Contents Materials Figures Literature Cited Abstract ChIP?chip combines
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