- ￥5000
- GeneTex
- 美国
- GTX60801
- 2025年07月16日
- ICC/IF, IP, Dot, ChIP assay
- Rabbit
- Species independent
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Species independent
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX60801
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
ICC/IF, IP, Dot, ChIP assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
5-Carboxylcytosine / 5-caC
- 抗体英文名：
5-Carboxylcytosine / 5-caC antibody
- 抗体名：
5-Carboxylcytosine / 5-caC 抗体
- 规格：
50 μg
ChIP analysis of 2 μg of J1 ES genomic DNA spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications using GTX60801 5-Carboxylcytosine / 5-caC antibody. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP'd DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control.
Dot blot analysis of 25 and 125 ng of the synthetic oligonucleotides containing different modified C-bases (indicated in red) using GTX60801 5-Carboxylcytosine / 5-caC antibody.
Dilution : 1:1,000
ICC/IF analysis of 293T cells overexpressing either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) using GTX60801 5-Carboxylcytosine / 5-caC antibody.
Dilution : 1:500
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文献和实验Yan JB et al., Mol Ther Oncolytics 2020 (PMID:32728616)
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
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