- ￥5800
- GeneTex
- 美国
- GTX60746
- 2025年07月15日
- WB, FACS, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human Alkaline phosphatase (intestinal) (AA: 397-458) expressed in E. Coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60746
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, FACS, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Alkaline phosphatase (intestinal)
- 抗体英文名：
Alkaline phosphatase (intestinal) antibody [3B10E7]
- 抗体名：
Alkaline phosphatase (intestinal) 抗体 [3B10E7]
- 规格：
100 μg
ELISA analysis of antigen using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
WB analysis of HL60 (1) and HepG2 (2) cell lysate using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].
WB analysis of HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].
FACS analysis of HepG2 cells using GTX60746 Alkaline phosphatase (intestinal) antibody [3B10E7].
Green : Alkaline phosphatase (intestinal)
Purple : negative control
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文献和实验Tao Bing et al., Advanced Science 2019 (Epub)
Bing T et al., Adv Sci (Weinh) 2019 (PMID:31179220)
Alkaline Phosphatase (EC 3.1.3.1)
bacteria, fungi, invertebrates, fish, and mammals (being located in many organs, including bone marrow, kidney, placenta, and intestinal mucosa), but have not been isolated from higher plants. The most commonly used alkaline phosphatases (AP
Conjugation of Antibodies to Alkaline Phosphatase
Alkaline phosphatase is coupled to immunoglobin G antibody in a one-step procedure using the homobifunctional reagent glutaraldehyde, which reacts with amino groups in the two proteins. The procedure is simple to perform and requires minimal
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
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