- ￥5800
- GeneTex
- 美国
- GTX60517
- 2025年06月29日
- WB, ICC/IF, FACS, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human RSK2 expressed in E. Coli.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60517
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS, ELISA
- 靶点：
RSK2
- 抗体英文名：
RSK2 antibody [4E10]
- 抗体名：
RSK2 抗体 [4E10]
- 规格：
100 μl
ICC/IF analysis of HepG2 cells using GTX60517 RSK2 antibody [4E10].
Green : RSK2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
WB analysis of human RSK2 (AA: 1-212) recombinant protein using GTX60517 RSK2 antibody [4E10].
ELISA analysis of antigen using GTX60517 RSK2 antibody [4E10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
FACS analysis of HepG2 cells using GTX60517 RSK2 antibody [4E10].
Green : RSK2
Red : negative control
WB analysis of HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate using GTX60517 RSK2 antibody [4E10].
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文献和实验Wholemount Immuno-flurescentWholemount Immuno-flurescent
as much extraembryonic membranes as possible. Puncture the amnion and in older embryos (>E10) puncture the head with a syringe or tungsten needle. 2. Fix in 4% paraformaldehyde (PFA) in PBS at 4o C overnight. 3. Wash twice for 5-10 minutes in PBS. 4. Wash
membranes as possible. Puncture the amnion and in older embryos (>E10) puncture the head with a syringe or tungsten needle. 2. Fix in 4% paraformaldehyde (PFA) in PBS at 4oC overnight. 3. Wash twice for 5-10 minutes in PBS. 4. Wash with 50% MeOH/PBS
b-Gal Staining of Murine Embryos
to make 0.2% Glutaraldehyde fixative, eg: add 0.2ml 25% Glutaraldehyde (EM grade) into 24.8ml fixing buffer. (fresh make every time) For E10 - E12 embryo - fix the embryo on ice for 10 minutes (with at least 20ml fixative); - rinse with PBS
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