- ￥5300
- GeneTex
- 美国
- GTX50274
- 2025年07月15日
- WB, ICC/IF, IHC-P, IHC-Fr
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of threonine 202 (F-L-T(p)-E-Y) derived from human ERK1.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX50274
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr
- 浓度：
1 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
ERK1 (phospho Thr202) + ERK2 (phospho Thr185)
- 抗体英文名：
ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody
- 抗体名：
ERK1 (phospho Thr202) + ERK2 (phospho Thr185) 抗体
- 规格：
100 μl
ICC/IF analysis of methanol-fixed HeLa cells using GTX50274 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody.
WB analysis of extracts from 293 cells untreated or treated with PMA for the indicated times using GTX50274 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody.
IHC-P analysis of human breast carcinoma tissue using GTX50274 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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文献和实验He ABH et al., Neurobiol Learn Mem 2019 (PMID:31521799)
Li HY et al., J Agric Food Chem 2019 (PMID:30661353)
Szu-Ting Lin et al., Cellular and Molecular Life Sciences 2015 (PMID:25596698)
Yinghao Yu et al., J Clin Med 2021 (PMID:34361981)
Bai-Chuang Shyu et al., Front Aging Neurosci 2021 (PMID:34744692)
Huang ACW et al., Neurobiol Learn Mem 2020 (PMID:32407964)
Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2
The action of ERK1 and ERK2 activity on the nuclear substrates requires crossing the nuclear envelope and the localization of phospho-ERK into the nucleus. The nucleo-cytoplasmic trafficking of ERK is therefore crucial for the correct
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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