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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human PECAM-1
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat, Bovine
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX15842
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS, IP, ELISA, IHC, Neutralizing/Inhibition
- 浓度:
1.06 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
CD31
- 抗体英文名:
CD31 antibody [HEC7]
- 抗体名:
CD31 抗体 [HEC7]
- 规格:
100 μg
ICC/IF analysis of THP-1 cells using GTX15842 CD31 antibody [HEC7]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC
FACS analysis of PBMC cells (green) using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% PFA
FACS analysis of BAEC cells using GTX15842 CD31 antibody [HEC7] compared to an isotype control (blue).
Dilution : 0.5 ug/test for 60 min at room temperature
Fixation : 2% PFA
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文献和实验CD31 分子 CD31 常用单克隆抗体或代号: SG134,TM3,HEC- 75;(PECAM、EndoCAM) 主要表达细胞: Pt,M,G,B,NK,Tsub [AS] 分子质量(kDa)和结构: gp140,血小板GPⅡa(IgSF) 功 能: 嗜同性或嗜异性(与CD38互为受体)粘附,炎症,En功能 CD31 Aka
Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas
is always required for different tissues and antigens. Using a low-power antigen retrieval technique, with appropriate dilution of antibodies, we successfully immunostained key antigens in the pancreas such as insulin, PDX-1, glucagon, cytokeratin, and CD31
) 3. BSA 4. DMEM 5. FCS 6. Antibiotic 7. Heparin 8. F12medium 9. Isoflourane 10. Ethonol 11. Dynabeads 12. anti-CD31antibody(BDPharmingen,catalognumber:550274) 13. M199 14. gelatin 二、设备 1. 离心机 2. 无菌培养橱
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