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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The immunogen used to generate this antibody corresponds to PEG
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX127286
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
0.71 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
PEG
- 抗体英文名:
PEG antibody
- 抗体名:
PEG 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
BSA and mPEG24 BSA were separated by 7.5% SDS-PAGE, and the membrane was blotted with PEG antibody (GTX127286) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验PEG Preparation of Plasmid DNA
PEG Preparation of Plasmid Plasmid isolated by this procedure can be used routinely
1、用超声波或冻融的方法使细胞破碎释放出病毒;2、慢慢搅动并加入NaCl终浓度是0.5mol/L,有助于病毒的沉淀,再等量加入10%PEG(一般使用的是PEG6000就可以了。如果对样品的纯度不是严格要求的话,可以建议使用Millpore公司的离心浓缩柱,Amicon系列的可以一次浓缩15ml样品。);3、4℃过夜或放置一段时间;4、8000/min离心30分钟,收集病毒沉淀;5、将病毒沉淀加入适量的pbs中,4℃过夜;6、10000r/min离心1小时,沉淀即为浓缩的病毒。还可以用梯度
as described above (5). 10. Wash the once briefly in 70% ethanol and air dry for 5 minutes. 11. Re-dissolve in water or TE (10ul per ml of original bacterial culture), add an equal volume of 13% PEG 8000, 800mM NaCl, mix and incubate on ice for 30 minutes
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