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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Full length Renilla Luciferase recombinant protein
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX125851
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-Wm
- 浓度:
1.14 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Renilla Luciferase
- 抗体英文名:
Renilla Luciferase antibody
- 抗体名:
Renilla Luciferase 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Renilla Luciferase antibody detects Renilla Luciferase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.
Green: Renilla Luciferase stained by Renilla Luciferase antibody (GTX125851) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Renilla Luciferase antibody detects Renilla Luciferase protein by Western blot analysis. Non-transfected (-) and Renilla Luciferase -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Renilla Luciferase antibody (GTX125851) at a dilution of 1:2000.
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文献和实验Delroisse J et al., Zool. Anz. 2017;269(2017)
Delroisse J et al., Open Biol 2017 (PMID:28381628)
Pecot J et al., Cell Rep 2016 (PMID:28009301)
Masuho I et al., J Biol Chem 2013 (PMID:23857581)
Treffers EE et al., PLoS Pathog 2023 (PMID:36848386)
Tessler M et al., Sci Rep 2020 (PMID:33082360)
den Hoed J et al., Sci Rep 2018 (PMID:30250039)
Gangadaran P et al., Oncotarget 2017 (PMID:29299117)
过程是第一天seeding,第二天换low serun的opti-MEM加lipofactamin,Renilla,和我的质粒过夜。第三天加compound,incubate一个小时以后加PMA,PHA,在incubate6个小时以后测量。Empty vector的读数和NF-kB的读数差很远,我想Transfection还是成功的。但是加了PMA和PHA的和不加的读数几乎没有区别。加了compound的和不加的也没有区别。 raw ratio 都在15左右。请高手赐教,不胜
The Split Luciferase Complementation Assay
substrate. This luminescence is then measured by a microplate luminometer. Amounts of the bait protein accumulated in the protoplasts can be estimated by Western blotting using an antibody that recognizes the amino-terminal fragment of Renilla luciferase
相关专题 在调控位点分析中,Luciferase主要采用的方法为:片段缺失、定点突变 ,流程如下所示。 图1 片段缺失分析 位点流程图 图2 定点突变分析位点流程图 荧光素酶报告基因检测法是转录调控研究中十分重要的实验手段。但其结果往往存在一定的误差。未解决这一问题,现普遍采用双荧光素酶报告基因检测法。在双荧光素酶报告基因测试中,将萤火虫荧光素酶作为实验报告基因,海肾荧光素酶作为对照报告
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